Objective :
@EN Systemic Lupus Erythematosus(SLE) is characterized by various autoantibodies to a variety of nuclear antigens. Certain extractable nuclear antigens(ENA) have been served as extremly useful aids in differentiation of clinical subset,
diagnostic
marker and in the detection of early forms of systemic rheumatic diseases. This study was to employ immunoblot to determine the prevalences of antibodies to ENA in SLE compares with immunodiffusion.
@ES Methods :
@EN Sera were obtained from 127 SLE patients. Antibodies to ENA were assessed by double immunodiffusion(DID0 and immunoblot method.
@ES Results :
@EN Using the immunblot method, the prevalences of antibodies to ENA were as follows: The antibody to Sm was 27%, UIRNP 33.6%, ro 41.8%, La 14%, ribosomal P 14% and Ku 4%. The prevalences of antibodies to ENA by DID were as follows : The antibody
to Sm
was 15%, RNP 24.5%, Ro 54.3% and La 9.4%.
@ES Conclusions :
@EN Compared with immunodiffusion, results using immunoblot showed greater sensitivity in the detection of autoantibodies to ENA in SLE.
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